How To Pour An Agar Plate Science Fair Biology Science Teacher While pouring the agar, open the petri dish lid as little as possible, hold it at an angle, and make sure the lid is kept directly over the petri dish. pour enough melted agar into each sterile plastic petri dish to cover 1 8" of the bottom. cover the lid of the petri dish immediately. place agar plates on a counter top to cool and set. With a nutrient agar bottle and petri dish, you can just melt and pour! whether it’s bacteria or fungi (or even something new), the students in your class will enjoy having a new world of life to explore. how to resources. stretch your supply budget further by pouring your own agar plates. it’s easy to do when you watch our step by step.
Tutorial How To Pour Agar Plates And Set Up A Bacterial Culture Interpreting plates. bacteria grow tremendously fast when supplied with an abundance of nutrients. different types of bacteria will produce different looking colonies, some colonies may be colored, some colonies are circular in shape, and others are irregular. If plates have been refrigerated, set them out and allow them to warm to room temperature. sterilize the loop. to sterilize the loop, hold the handle with a pot holder and place the tiny looped wire in a flame until it turns bright red. allow the loop to cool for 3 5 seconds before touching the collection area. Pour into plate until it covers the bottom, approximately 25 ml (see video below). after several hours to overnight, return the plates to the plastic sleeve they came in or place them in a plastic bag. label the bag with the media type and the date, and store upside down in a refrigerator. plates will keep refrigerated for 4 6 weeks. 8 tips for pouring perfect agar plates every time. 1. use a recipe. make up the medium according to the recipe, then add the desired amount of agar powder (normally about 1% w v) and stir. if you autoclave without stirring, with the agarose still floating on top of the liquid, you get an agarose cake in the medium.
How To Pour Agar Plates Youtube Pour into plate until it covers the bottom, approximately 25 ml (see video below). after several hours to overnight, return the plates to the plastic sleeve they came in or place them in a plastic bag. label the bag with the media type and the date, and store upside down in a refrigerator. plates will keep refrigerated for 4 6 weeks. 8 tips for pouring perfect agar plates every time. 1. use a recipe. make up the medium according to the recipe, then add the desired amount of agar powder (normally about 1% w v) and stir. if you autoclave without stirring, with the agarose still floating on top of the liquid, you get an agarose cake in the medium. Assembly. measure out 1.6 g of agar agar and 200 ml water. mix them together with a whisk or fork in a large microwave safe bowl. heat the solution in the microwave on high for 30 seconds. remove to a heat safe surface using a hot pad or oven mitts, stir, and return to the microwave for 30 seconds. New life science. browse the latest tools and resources for life sciences at carolina. carolina biological supply has everything you need to complete your classroom life science activities and experiments.